Multiple phosphorylation sites in RGS16 differentially modulate its GAP activity
نویسندگان
چکیده
منابع مشابه
Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sites.
The enzymic activity of the mammalian pyruvate dehydrogenase complex is regulated by the phosphorylation of three serine residues (sites 1, 2 and 3) located on the E1 component of the complex. Here we report that the four isoenzymes of protein kinase responsible for the phosphorylation and inactivation of pyruvate dehydrogenase (PDK1, PDK2, PDK3 and PDK4) differ in their abilities to phosphoryl...
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MSK1 (mitogen- and stress-activated protein kinase) is a kinase activated in cells downstream of both the ERK1/2 (extracellular-signal-regulated kinase) and p38 MAPK (mitogen-activated protein kinase) cascades. In the present study, we show that, in addition to being phosphorylated on Thr-581 and Ser-360 by ERK1/2 or p38, MSK1 can autophosphorylate on at least six sites: Ser-212, Ser-376, Ser-3...
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The 68,000, 145,000, and 200,000 dalton subunits of neurofilaments from rat spinal cord and brain stem were shown by chemical phosphate analysis to contain about 3, 9, and 22 mol of phosphate/mol of polypeptide, respectively. All three subunits contained phosphoserine while the 145,000 dalton polypeptide also contained phosphothreonine. The high phosphate content of the 200,000 dalton polypepti...
متن کاملMultiple Sites in αB-Crystallin Modulate Its Interactions with Desmin Filaments Assembled In Vitro
The β3- and β8-strands and C-terminal residues 155-165 of αB-crystallin were identified by pin arrays as interaction sites for various client proteins including the intermediate filament protein desmin. Here we present data using 5 well-characterised αB-crystallin protein constructs with substituted β3- and β8-strands and with the C-terminal residues 155-165 deleted to demonstrate the importanc...
متن کاملMultiple Sites in aB-Crystallin Modulate Its Interactions with Desmin Filaments Assembled In Vitro
The b3and b8-strands and C-terminal residues 155–165 of aB-crystallin were identified by pin arrays as interaction sites for various client proteins including the intermediate filament protein desmin. Here we present data using 5 well-characterised aB-crystallin protein constructs with substituted b3and b8-strands and with the C-terminal residues 155–165 deleted to demonstrate the importance of...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 2001
ISSN: 0014-5793
DOI: 10.1016/s0014-5793(01)02757-0